๐Ÿ„ MycologyLog.com by LACampanelli LLC. OSD + WBS Hydrated Grain ยท Sous-vide Conditioning
OSD + WBS Hydrated Grain Recipe

Ingredients & Ratios

Ingredient Unit Amount % of Total*
OSD oz 3 9.9%
WBS oz 12.2 40.4%
Sugar tsp 1/4 trace
Water oz 15 49.7%
Total oz โ‰ˆ 30.2 100%

*Percentages are approximate, based on total mix weight; sugar mass is very low and treated as trace.

Estimated Hydration: 15 oz water รท 30.2 oz total โ‰ˆ 50% moisture
Suitable as a pre-conditioned grain + sawdust mix prior to full sterilization.

Method

  1. Weigh out 3 oz OSD and 12.2 oz WBS into a large, high-temp rated bag.
  2. Add 1/4 tsp sugar to the dry mix.
  3. Pour in 15 oz water and mix thoroughly so the OSD and WBS are evenly hydrated and there is still some free water in the bag.
  4. Vacuum-seal the bag for about 10 seconds while free water is still visible. The bag is large enough to handle a 10-second seal โ€” possibly a little longer; you can try 12 seconds next time if the seal looks solid.
  5. Place the sealed bag into a sous-vide bath and hold it at your desired conditioning temperature for the planned time.
  6. After the sous-vide step, allow the bag to cool enough to handle safely.
  7. Run your usual sterilization cycle (e.g., pressure sterilizer/autoclave), using your standard time and temperature for grain spawn.
  8. Let the bag cool completely to room/inoculation temperature, then inoculate under clean conditions according to your normal workflow.

โ„น Process Notes

Mixed together in one bag and vacuum-sealed for 10 seconds while there was still free water present. The bag is large enough to handle a 10-second seal โ€” maybe even a little longer; try 12 seconds next time.

The purpose of the sous-vide step prior to sterilization is twofold:

  • To hydrate the grain/OSD, serving as a substitute for the traditional overnight soak.
  • To lightly sanitize the grain before the full sterilization process.